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Understanding HCV Real-Time PCR Kits

There are all sorts of real-time PCR kits that are widely available to test an array of viruses that cause different disease conditions. If you are looking for quality HCV real time PCR kits, you have to find a company that only offers the best possible products to choose from. With the right PCR kit with you, you know that you will be getting user-friendly, flexible, and reliable agents that work well with your testing volumes, instruments, and laboratory organization.

There are different protocols that makers of HCV real-time PCR kits use. The use of a single amplification and extraction protocol for all PCR kits enables testing an array of RNA or DNA pathogens on the same plate. The use of rapid and simple protocols allows you to get the results of the test in just a matter of 90 minutes starting with extraction going to amplification.

The use of rigorous validation criteria is one of the benefits of using only quality HCV real time PCR kits. Having this benefit means that you will have positive controls that help to evaluate how efficient your amplification and extraction steps are. Flexibility is a crucial component in the PCR kit that you choose. A flexible kit is one that works well with the majority of extraction systems like silica columns and magnetic beads. They should be compatible with all possible thermocyclers too.

Of course, a quality HCV PCR kit should be easy to use. It must have ready-to-use reagents. When it comes to an amplification reaction mix that you will be getting should have all the right probes, primers, and master mix necessary to run the qPCR. There is also the ability to utilize a rapid one-hour amplification protocol. Also, you have to go with a PCR kit that is validated by recognized international reference laboratories.

When it comes to HCV real-time PCR kits, the utilization of real-time PCR techniques is a must. When it comes to regular PCR methods, and end-point analysis is used to detect the amplicon or amplified DNA. In the application of real-time PCR, the accumulation of the products being amplified is measured, especially as there is a real-time progression in the reaction. Product quantification essentially happens after every cycle.

The qPCR workflow, on the other hand, utilizes more or less the same steps as those utilized in PCR in real-time. The first step is setting up of these amplification reactions using PCR reagents as well as custom or unique primers. The use of PCR instruments in real-time is then crucial for running the reactions. The use of proper instrument software from the company is vital for analyzing the data gathered.

The detection of PCR products in real-time allows each reaction well to include a fluorescent reporter molecule. It then results in an increase in fluorescence with the rising amount of the DNA product. The fluorescence chemistry components that are used for this purpose include sequence-specified probes or primers that are labeled fluorescently as well as DNA-binding dyes. As amplification takes place, fluorescence signals are monitored using unique thermal cyclers that come with fluorescence detection modules. The fluorescence measured is more or less the same with the total amplicon amount. Over time, changes in fluorescence are utilized to get the amount of amplicon that each cycle produces.

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